Our team combines genetics, biophysics, physiology & behavior to understand how sensory inputs are integrated in the spinal cord during development and active locomotion. Our lab discovered that neurons contacting the cerebrospinal fluid (CSF) in the spinal cord are mechanoreceptors detecting curvature of the spinal cord and CSF flow, which modulate the activity of spinal neurons controlling locomotion and posture. We use the transparent zebrafish larva to implement optical methods for manipulating and monitoring neuronal activity in motion. Our work aims to unravel the mechanisms by which interoceptive sensory inputs are integrated throughout life to form the spinal cord, and insure homeostasis in the mature stages. One critical effort lies in testing the results we obtained in zebrafish in humans.

research

Neuromodulation in hindbrain and spinal cord

Arousal locomotion is strongly modulated by our inner physiological states. This spontaneous exploratory locomotion reflects the excitability of motor circuits in the spinal cord as well as descending commands from the brain, in particular from the hindbrain. The underlying mechanisms controlling the occurrence of spontaneous locomotion and its natural variability among animals and across physiological states within one animal are not well understood. We are interested in probing neuromodulatory pathways in the hindbrain and spinal cord for setting the frequency of occurrence of locomotion in the context of circadian rhythm, inflammation and feeding.

Modulation of motor circuits via the cerebrospinal fluid

The classical view of spinal cord physiology relies on the fact that motor functions are carried by ventral spinal cord while dorsal spinal cord integrates sensory inputs from the periphery. Up to recently, there was no evidence that the vertebrate spinal cord carried itself sensory functions. Our team has shown evidence for a central sensory motor loop localized in the spinal cord and modulating circuits underlying locomotion and posture. We have evidence that the morphology and molecular markers of this central sensory system is conserved in the mammalian spinal cord.

Mechanosensory feedback to the spinal cord during active locomotion

The contribution of mechanosensory feedback to active locomotion and the nature of underlying spinal circuits remain elusive. We investigate how mechanosensory feedback shapes active locomotion in the zebrafish larva. We find that mechanosensory feedback enhances the recruitment of motor pools during active locomotion. We show that inputs from glutamatergic mechanosensory neurons increase locomotor speed by prolonging fast swimming at the expense of slow swimming during stereotyped acoustic escape responses. Altogether, our efforts reveal the basic principles and circuit diagram underlying speed modulation by mechanosensory feedback in the vertebrate spinal cord.

people

events

01/04/2017
April 1st, 2017: Kevin moves to Columbia University

http://sklad.cumc.columbia.edu/jessell/

16/03/2017
March 16th, 2017: Kristen moves to the Bormuth lab in UPMC!

http://www.labos.upmc.fr/ljp/?vbormuth&lang=en

01/03/2017
March 1st, 2017: Urs starts in Harvard University

http://cohenweb.rc.fas.harvard.edu/

March 1st, 2017: Lydia starts in Harvard university

http://hsci.harvard.edu/people/iain-drummond-phd

09/02/2017
Lydia's article is accepted in Scientific Reports : Congratulations !

http://www.nature.com/srep/

07/02/2017
Claire is awarded the 2017 prize from the Fondation Scientifique pour l Education et la Recherche

Download document | http://www.lemonde.fr/sciences/article/2017/02/06/claire-wyart-illumine-le-mouvement_5075364_1650684.html

21/11/2016
Claire is awarded in 2016 the New York Stem Cell Foundation Innovator in Neuroscience Award

https://nyscf.org/news/nyscf-press-releases/item/2081-nyscf-announces-the-six-members-of-the-2016-class-of-nyscf-robertson-investigators

20/10/2016
Claire is a 2016 awardee of the EMBO-Young Investigator Program

http://www.embo.org/news/press-releases/2016/twenty-five-life-scientists-join-embo-young-investigator-network

20/08/2016
Jeff's article is accepted in Current Biology : Congrats !

Download document | http://www.cell.com/current-biology/fulltext/S0960-9822(16)30938-1

04/07/2016
Jenna & Kris's article is accepted in Current Biology: Congrats !

Download document | http://www.cell.com/current-biology/fulltext/S0960-9822(16)30676-5

23/05/2016
Oscar from the Emiliani lab publishes 3D holographic method for optogenetic applications : Congrats!

Download document | http://www.nature.com/ncomms/2016/160616/ncomms11928/abs/ncomms11928.html

22/02/2016
Urs and Andy's article is accepted in Nature Communications: Congrats !

Download document | http://www.nature.com/articles/ncomms10866

15/12/2015
Kevin's article is accepted in Current Biology : Congrats !

Download document | http://www.cell.com/current-biology/fulltext/S0960-9822(15)01224-5

01/09/2014
Lydia's article is published in Frontiers in Neuroanatomy !

Download document | http://journal.frontiersin.org/article/10.3389/fnana.2014.00026/full

01/04/2013
Olivier's article is out in Frontiers in Neural Circuits

Download document | http://journal.frontiersin.org/article/10.3389/fncir.2013.00107/full

outreach