The publications from the Wyart lab are accessible on our website here: http://wyartlab.org/publications
Find out recent news on twitter: Claire's @ClaireWyart and the lab @wyartlab
Our lab combines genetics, biophysics, physiology & behavior to understand how sensory inputs are integrated in the spinal cord during development and active locomotion. The lab discovered that neurons contacting the cerebrospinal fluid (CSF) in the spinal cord are mechanoreceptors detecting curvature of the spinal cord and CSF flow, which modulate the activity of spinal neurons controlling locomotion and posture. We use the transparent zebrafish larva to implement optical methods for manipulating and monitoring neuronal activity in motion. Our work aims to unravel the mechanisms by which interoceptive sensory inputs are integrated throughout life to form the spinal cord, and insure homeostasis in the mature stages.
Three permanent researchers in the team:
Dr. Claire Wyart graduated from the Ecole Normale Supérieure Ulm in 2000. Under the supervision of Laurent Bourdieu and Didier Chatenay, she obtained her PhD in biophysics and neuroscience from the University of Strasbourg and moved to University of California in Berkeley for her postdoc. In the lab of Udi Isacoff, she developed optical techniques to control activity of neurons remotely in vivo (optogenetics). Dr. Wyart is EMBO Young investigator & EMBO full member, New York Stem Cell Foundation (NYSCF) Robertson Investigator, FENS-Kavli Network of Excellence (FKNE) Scholar and Board member. Commitment to science outreach and training in science: https://zenith-etn.com; https://en.adioscorona.org
Dr. Yasmine Cantaut-Belarif is a permanent researcher at CNRS since 2020. She did her PhD with Antoine Triller in the Ecole Normale Supérieure Ulm. During her postdoctorate fellowship in the Wyart's team between 2017 and 2020, she demonstrated the role of the Reissner fiber in the formation of the axis. Her research now tackles the molecular mechanisms by which the fiber can control the straightness of the body axis.
Prof. Hugues Pascal-Moussellard is a MD PhD and surgeon in charge of the orthopedic department of the Pitié-Salpêtrière hospital in Paris 13. He conducts with Laura Marie Hardy, Thomas Courtin and Prof. Alexis Brice a project aiming to decipher mutations associated with idiopathic scoliosis in humans.
Arousal locomotion is strongly modulated by our inner physiological states. This spontaneous exploratory locomotion reflects the excitability of motor circuits in the spinal cord as well as descending commands from the brain, in particular from the hindbrain. The underlying mechanisms controlling the occurrence of spontaneous locomotion and its natural variability among animals and across physiological states within one animal are not well understood. On one end, we are interested in probing neuromodulatory pathways in the hindbrain and spinal cord for setting the frequency of occurrence of locomotion in the context of circadian rhythm, inflammation and feeding. On the other, we investigate how neuromodulation can influence morphogenesis.
The classical view of spinal cord physiology relies on the fact that motor functions are carried by ventral spinal cord while dorsal spinal cord integrates sensory inputs from the periphery. Up to recently, there was no evidence that the vertebrate spinal cord hosts sensory cells conserved throughout vertebrates. Our team has shown evidence for a central sensory motor loop localized in the spinal cord and modulating circuits underlying locomotion and posture. We have evidence that the morphology and molecular markers of this central sensory system is conserved in the mammalian spinal cord. This axial sensory system modulates locomotion, posture and morphogenesis.
The contribution of mechanosensory feedback to active locomotion and the nature of underlying spinal circuits remain elusive. We investigate how mechanosensory feedback shapes active locomotion in the zebrafish larva. We find that mechanosensory feedback enhances the recruitment of motor pools during active locomotion. We show that inputs from glutamatergic mechanosensory neurons increase locomotor speed by prolonging fast swimming at the expense of slow swimming during stereotyped acoustic escape responses.
Moreover we have evidence that sensory integration is critical throughout life for growing and maintaining a straight body axis.
Altogether, our efforts reveal the basic principles and circuit diagram underlying the modulation of movement, posture and morphogenesis by adjusted mechanosensory feedback in the vertebrate spinal cord.
Celine did her undergraduate studies in UC Berkeley and spends one year in Paris working on the Reissner fiber before starting grad school !
Yasmine Cantaut Belarif obtained her PhD from the Biological Department of the Ecole Normale Supérieure and University Pierre et Marie Curie (UPMC) in 2015 where she worked on interactions between immune cells and nervous system at the nanoscopic scale on proteins from inhibitory synapses in the dorsal spinal cord. After a postdoc in the lab in which she discovered that the Reissner fiber is critical for straightening the body axis, she obtained in 2020 a permanent researcher position in CNRS. In 2021, she successfully obtained the competitive French grant ANR JCJC to support the scientific autonomy of young researchers in France. Find more about Yasmine on Twitter here !
Yasmine studies the roles of scospondin and the Reissner fiber in axon pathfinding and the development of the spinal cord in zebrafish.
Martin received his PhD in Neurosciences in 2013 from the University of Buenos Aires in Argentina. In the Lab of Dr. M. Eugenia Pedreira, Martin studied the role of the GABAergic system in the different phases of memory formation, focusing in the extinction memory in the crab. His PhD work combined pharmacology and immunohistochemistry. Then he moved to learn electrophysiology and calcium imaging under the tutelage of Dr. Lidia Szczupak to study the role of a recurrent inhibitory circuit in sensorymotor networks in the leech nervous system. Martin joined our lab in 2018 and participated already in 3 important projects. First, he showed with Ming Yue Wu that cerebrospinal fluid contacting neurons in the rostral spinal cord control posture during fast escape responses by projecting onto the soma and dendrites of occipital motor neurons as well as onto the descending axon of command neurons in the brainstem. Second, he discovered the location of the mesencephalic locomotor region (MLR) in larval zebrafish and uncovered how the MLR recruits V2a reticulospinal neurons in the medulla. Finally, Martin was instrumental in a key collaboration with Filippo Del Bene and Gokul Rajan to compare the mechanisms underlying continuous versus discrete locomotion in Danionella cerebrum and Danio rerio. Find more on Twitter about Martin here !
His future projects involve to decipher the role of the MLR in navigation and goal-directed actions. Martin has also setup collaborations with geneticists to investigate the contribution of critical genes involved in Parkinson's disease in humans in the structure and function of the motor circuits in zebrafish.
Clothilde did her first two years of bachelor at University of Lille with a major in Cell biology. After a gap year in Australia, she integrated the “Magistère Européen de Génétique” (University of Paris) for her last year of Bachelor, followed by her Master. There, she had the opportunity to do a 4-months internship in Porto (Portugal) in the team of Nuno dos Santos, where she learned techniques of molecular biology. Her long-term interest has always been the biology of Development, but she recently discovered with pleasure the world of Neurosciences. This year, she therefore followed the Pasteur’s course “Development & Plasticity of the Nervous System”. She’s happy to integrate the team for a 6-months internship, as part of her last year of Master, in order to study the molecular mechanisms underlying body axis straightening in the embryo.
Faustine is a software/research engineer who joined the lab in November 2020, after graduating from a master in Bioengineering specialized in Computational neurosciences (EPFL, Switzerland) as well as a second master in AI (KU Leuven, Belgium). Her interest is transdisciplinary and she wants to use computational methods to better understand the neuronal mechanisms underlying behavior. Faustine is currently analyzing the calcium activity of neurons in the hindbrain of larval zebrafish performing the optomotor response, and investigating directional neuronal communication with the aim of linking the neural activity to the locomotor behavior.
Joana received her PhD in Immunology in 2017 from Imperial College London, UK, studying the role of NKG2D in models of liver inflammation and tumorigenesis in the Lab of Dr Nadia Guerra. Passionate about collaboration, innovation and interdisciplinary in science, she then moved to working in research and project management in international universities and research funding agencies.
Joana is now the project manager of the ZENITH European training programme (https://zenith-etn.com), an exciting interdisciplinary neuroscience-focused PhD network, that aims to train a new generation of neuroscientists in cutting-edge approaches that bridge biology, physics, mathematics and computer science to uncover the mysteries of brain formation and function.
Agnès received a technician diploma in 2017. She entered EBI (Ecole de Biologie Industrielle), an engineering school based in Cergy, and graduated in 2021, with the specialization Research and Application. After an end-of study internship in developmental biology, working on C. elegans, she joined the lab in December 2021 as engineer. Her project consists in developing transgenic lines of zebrafish. Apart from biology, Agnès is passionate about music: choir, piano, flute, and loves playing rugby !
Xinyu Jia is a first year grad student who obtained a master in Optics as well as a master in Neuroscience in University of Edinburgh in Scotland. Xinyu was selected among >100 students for the ZENITH European training programme (https://zenith-etn.com).
Xinyu's PhD project focuses on investigating the function of descending command neurons to sustain distinct locomotor episodes using 3D optogenetics in vivo. She will be co-advised by Claire Wyart and Dimitri Tanese from the Emiliani lab in the vision institute.
After entering med school in Lyon in 2015, Mathilde joined the Ecole de l’Inserm Liliane Bettencourt in 2017 to follow a double joint path, in order to achieve an early formation to research along her medical studies. Her long-term interest on neural and muscular comprehension lead her to her first research internship in Lyon at Neuromyogene Institute on understanding the physiological and pathological regeneration of the muscle, and to her second internship in University of Ottawa, on the sensorimotor integration in the electric fish. Taking a gap year in her medical studies, she started a second year of master in Cellular and Integrated Neurosciences at Sorbonne University in September 2018. She joined Wyart’s team in October 2018 to work on circuits driving exploratory locomotion in Zebrafish.
BIOGRAPHY. After a two-years technical degree obtained in 2014 in bio-analysis and controls, Hugo started the Magistère Européen de Génétique (Paris) and is doing his last year in this program. Through the Magistère, Hugo learnt different molecular methods, especially by doing two internships in Edinburgh and Chicago, in Dr. Goodrich and Pr. Holmgren teams, respectively.
PROJECTS. A RNA-seq performed on cerebrospinal fluid-contacting neurons (CSF-cNs) highlighted different interesting genes expressed in these cells. Hugo will focus on 8 of these genes, encoding for proteins or peptides secreted in CNF-cNs, and will investigate their functional roles in zebrafish, in collaboration with Feng QUAN.
Olivier Mirat graduated from a computer science master program before joining the lab. He created the tracking software ZebraZoom1.0 and subsequent improvements. Now working as freelance, he often helps people in the lab.
Zebrafish larvae swim as discrete bouts lasting for a couple of hundreds of milliseconds every few seconds. Their movements are very fast. Olivier generated a tracking software combined with a brain machine interface to implement automated categorization of every swim bout. His algorithms have changed the way we process and analyse locomotion in our lab.
BIOGRAPHY. Adeline received her PhD in Neuroscience in 2014 at Aix-Marseille University. During her PhD, she combined electrophysiological and labelling approach to characterize in mouse brainstem CSF-cNs: a new sensorial system through PKD2L1 channels. Then she improved her training by learning 2-photon calcium imaging at UTHSCSA, Texas where she studied the plasticity of presynaptic GABABreceptors induced by change in synaptic activity.
PROJECTS. Adeline's project aims to record from CSF-cNs in vivo in zebrafish larvae where she will combine calcium imaging and electrophysiology in different genetic models. In addition, Adeline will investigate the physiological roles of CSF-cNs in mammals. Her project is supported by the ERC starting grant.
Hugues is M.D. Ph.D., professor at the university Pierre and Marie Curie Paris-6 and head of the orthopedics department of the Pitié-Salpêtrière hospital. He did his initial studies in Marseille and was trained by Catonnet in Martinique at the beginning of his career.
Hugues wants to understand how physiological parameters covary and how we could best treat patients after injury. He has a master mind project aiming to record from all physiological parameters soon after spinal cord injury.
BIOGRAPHY. Julian started working in Philippe Ravassard's team in ICM after receiving his technician diploma in 2010. He specialized in cloning and molecular biology and joined the ICM vectorology platform in 2014. He developed ovo-transfert to clean all transgenic mice lines of the institute. In 2015, he joined the Pasteur Institute in Jerome Gros's team in order to establish quails transgenics lines and promote the quail as a new experimental model. Early 2017, Julian joined C.Wyart's team in 2017 as an Engineer, in order to help the team achieve its goals with his precious experience in molecular biology, transgenesis and animal experimentation.
PROJECTS. Julian is multi-tasking ! He develops tools using CRISPR/Cas9 technologies to generate new zebrafish gal4 transgenic lines via knock-in as well as to kill cells in vivo with a tight control of space and time. He performs as well fluorescence activated cell sorting combined with RNAseq on diverse sensory cells from zebrafish larva.
Gautam Sridhar joined the Wyart Lab after finishing his MSc in Electrical Engineering at ETH Zurich. Gautam’s interests in machine learning translate to applying such methods to investigate the structure in the locomotion of larval zebrafish. As part of the ZENITH interdisciplinary training network, he will be supervised primarily by Dr. Claire Wyart in investigating the pattern of zebrafish locomotion in the presence of various stimuli and contexts. He will further work with Dr. Moritz Grosse-Wentrup at University of Vienna to investigate causal links between characteristic behaviour and neuronal activity.
Claire Wyart did her undergrad studies in the Ecole Normale Supérieure Ulm between 1996 and 2000. She joined the biophysics lab of Didier Chatenay for her PhD between 2000 and 2003 in the Institute of Physics in the University of Strasbourg. During her PhD, Claire developed novel methods for controlling the architecture of neuronal networks in vitro and demonstrated mechanisms underlying the emergence of spontaneous activity. After one year of teaching in Tibetan schools, she joined UC Berkeley for her postdoctoral fellowship in the labs of Prof. Noam Sobel and Ehud Isacoff between 2005 and 2010. She developed optogenetic methods in vivo by taking advantage of the transparency of the zebrafish larva. In this small vertebrate model, Claire used optogenetics to study sensory-motor integration, analyzing the processing of visual, mechanosensory and chemosensory pathways modulating locomotion. Since 2011, Claire started her team in Brain and Spinal cord Institute (ICM) with the support of the ATIP / Avenir from Inserm and CNRS with the Fondation Bettencourt-Schuller, a chair of excellence from the Ecole des Neurosciences de Paris (ENP) and the emergence programme of the City of Paris. Subsequently, her team received support from the European Research Council (ERC) Starting Grant in 2012, the Human Frontier Science Program (HFSP) research grant in 2013, and a National Institute of Health (NIH) research grant from 2014. She became an EMBO-Young Investigator (EMBO-YIP) and received the New York Stem Cell Foundation (NYSCF) Robertson Innovation in Neuroscience award in 2016. She received the award from the Foundation for Scientific Education and Research (FSER) in 2017.
PROJECTS. Claire is interested by deciphering thel neuronal circuits that integrate information from the brain, from the periphery and from internal cues in order to modulate locomotion and posture as a function of our inner physiological states. Her strategy is to take advantage of the transparency of zebrafish larva to develop methods for monitoring and manipulating neuronal activity during behavior. The lab now combines functional studies with molecular profiling to identify cell identity underlying functional diversity of neurons in sensory-motor integration. A major emphasis of the lab focuses on the investigation of GABAergic sensory neurons interfacing the cerebrospinal circuits with motor circuits in the vertebrate spinal cord. Another focus of the lab lies in understanding the functional connectivity between neurons across the hindbrain and spinal cord using functional population calcium imaging. The third interest of the lab lies in the role of mechanosensory feedback from the periphery in shaping the dynamics of active locomotion. Our main collaborators include Mario Chavez in ICM, Filippo Del Bene in Curie Institute, Herve Tostivint in the Museum National d’Histoire Naturelle (MNHN).
Congrats to Faustine who got selected for the Qbio Neurophysics of Locomotion course in KITP!!
G. Reddy: A novel method to unbiasedly identify sequences of actions in complex motor patterns !
Congrats to Adeline & Maha showing that vestibular stimuli elicit massive glial calcium wave !
Congrats to Yasmine who got the ANR JCJC Young Researcher Grant !!
Congrats to Martin & Ming Yue to reveal the role of CSF contacting neurons in posture !
Olivier Thouvenin shares an automated method for analyzing flow and cilia motility !!
Thank you to the Fondation Bettencourt Schueller (FBS) for supporting our work on motor control !
Yasmine Cantaut-Belarif: Adrenergic activation influences the signal from the Reissner fiber
Congrats to Yasmine Cantaut-Belarif who got the national exams from CNRS and INSERM !
Thank you to Fondation pour la Recherche Médicale (FRM) for supporting our team !
Check out the latest version of ZebraZoom to monitor kinematics and posture in fish!
The Zenith European Training Network has selected students !
Congrats to Adna and Charlotte: A calibrated toolbox for in vivo optogenetics in zebrafish !
Congrats Adeline & Yasmine: the Reissner fiber is required to detect spinal curvature !
Modelling the flow of cerebrospinal fluid in the central canal!
Innate immunity at the CSF interface: how do sensory neurons fight infections in the CNS !
Claire Wyart is elected EMBO Member !
An article on morphogenesis and sensory function : how do microvilli contribute to sensing!
Hindbrain circuits underlying locomotion : focus on the inhibitory Eng1 brainstem neurons!
Check out single channel recordings IN VIVO: PKD2L1 & mechanotransduction !
HFSP research grant for the team: Investigation of signalling in the CSF in fish & mouse !
Postdoc fellowships for Olivier, Martin and Yasmine: Congrats!
Movie on the team : What is happening in the mind of scientists...
Congrats to Adna Dumitrescu for her travel grant : good luck now for your experiments in Chicago!
Congrats to Yasmine Cantaut-Belarif, PL Bardet for the Big Brain Theory grant from ICM !!!
Congrats to Martin Carbo-Tano for the Prestige fellowship!
July 2017: Laura Desban obtains a fourth PhD year from the FRM: congrats !
Our review is out on CSF-contacting neurons in Journal of Neurogenetics !
Mechanosensory feedback enhances speed of locomotion via a novel circuit !
April 2017: Kevin moves to Columbia University
March 2017: Kristen moves to the Bormuth lab in UPMC!
March 2017: Urs starts in Harvard University
Lydia starts in Harvard university
A study on neurosecretory properties and morphology of neurons contacting the CSF!
Claire is awarded the 2017 prize from the Fondation Scientifique pour l Education et la Recherche
Claire is awarded the 2016 New York Stem Cell Foundation Innovator in Neuroscience Robertson Award
Claire is a 2016 awardee of the EMBO-Young Investigator Program
How do neurons contacting the CSF project on fast locomotor circuits in the spinal cord ?
Check out the botulinum toxin light chain to silence vesicular release in vivo !
3D holographic method for optogenetic applications with the Emiliani lab!
First in vivo demonstration of the mechanosensory function of neurons contacting the CSF !
Identification of the V0-v as targets of neurons contacting the CSF in the spinal cord !
Conservation of neurons contacting the cerebrospinal fluid in mouse, macaque and zebrafish !
Congrats to Olivier Mirat: Development of the first version tracking algorithm ZebraZoom !
Wyart Lab
Spinal Sensory Signaling
I.C.M.
47, bld de l'hopital
Paris 75013 - FRANCE